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変異型大腸菌を用いた大気圧直流プラズマジェットによる殺菌機構の研究
◎李 鉀・瀧口昌樹・坂井奈津子・渡辺正人・堀田栄喜・和地正明(東京工業大学)
Mechanisms of plasma-induced microbial inactivation have commonly been studied with physicochemical techniques. In this study, Escherichia coli K-12 BW25113 and its ΔrecA, ΔuvrA, ΔrpoS and ΔsoxS mutants are employed to discriminate effects of DNA damage, UV photons, reactive oxygen species and environmental stress produced in an atmospheric-pressure DC plasma jet. With the help of optical emission spectroscopy, this biological method exploits the fact that reactive oxygen species, especially long-lying radical like O3 and O2 (1∆g), are playing a main role in inactivation process.